Education

• B.S. in Agriculture, Shandong Agricultural University, Shandong Province, P.R. China

• Ph.D. in Agricultural and Environmental Chemistry, University of California at Davis, USA

• Post-doctoral fellow, University of California at Berkeley, USA

Areas of Research

Research in this laboratory focuses on environmental biochemistry and biotechnology.  The goal of our research is to minimize the risks of pollutants and create high value products.  Topic areas include proteomics and metabolomics, biodegradation and biosynthesis, insect nutriproteomics and control, analytical/environmental chemistry and fate of pollutants and pesticides. 

Instruction

• Environmental Biochemistry (MBBE412, 3 credits- lecture course)

• Advanced Laboratory Techniques (MBBE687, 3 credits- lecture/lab course).

Professional Societies and Affiliations

• American Chemical Society

• American Association for the Advancement of Science

• USDA W-1045 technical committee

• USDA multi-regional project, S-1007, "The Science and Engineering for a Biobased Industry and Economy"

• USDA IR-4 program

• UH Water Resources and Research Center

• Molecular Biosciences and Bioengineering Graduate Program, UH-Manoa

• Food Science Graduate Program, UH-Manoa

• Nutrition Graduate Program, UH-Manoa

SELECTED PUBLICATIONS

148.  Hennessee, C.T.; Seo, J.-S.; Alvarez, A.M.; Li, Q.X.  2008.  Isolation and characterization of five new polycyclic aromatic hydrocarbon (PAH)-degrading Mycobacterium species isolated from Hawaiian soils:  Mycobacterium crocinum sp. nov., Mycobacterium pallens sp. nov., Mycobacterium rutilum sp. nov., Mycobacterium rufum sp. nov., and Mycobacterium aromaticivorans sp. nov.  Int J Syst Evol Microbiol. In press

135.  Wang, D.; Atkinson, S.; Hoover-Miller, A.; Shlever, W.; Li, Q.X. 2008.  Simultaneous use of gas chromatography/ion trap mass spectrometry - electron capture detection to improve the analysis of bromodiphenyl ethers in biological and environmental samples.  Rapid Commun. in Mass Spectrom. 22:647-656.

134.  Wang, D.L.; Miao, X.-S.; Li, Q.X.  2008.  Analysis of organochlorine pesticides in coral (Porites evermanni) samples by using accelerated solvent extraction and gas chromatography/ion trap mass spectrometry.  Arch. Environ. Contam. Toxicol. 50:211-218.

126.  Seo, J.-S.; Keum, Y.-S.; Harada, R.M.; Li, Q.X.  2007.  Isolation and characterization of bacteria capable of degrading polycyclic aromatic hydrocarbons (PAHs) and organophosphorus pesticides from PAHs-contaminated soil in Hilo, Hawaii.  J. Agric. Food Chem.  55(14):5383-5389.

122.  Lee, S.-E.; Seo, J.S.; Keum, Y.-S.; Lee, K.-J.; Li, Q.X.  2007.  Fluoranthene metabolism and associated proteins in Mycobacterium sp. JS14.  Proteomics 7(12):2059-2069.

120.  Seo, J.S., Keum, Y.S., Hu, Y., Lee, S.E., Li, Q.X. 2007. Degradation of phenanthrene by Burkholderia sp. C3: initial 1,2- and 3,4-dioxygenation and meta- and ortho-cleavage of naphthalene-1,2-diol. Biodegradation 18:123-131.

118.  Keum, Y.-S.; McQuate, G.T., Li, Q.X.  2007. Synergists isolated from cade oil for the parapheromone a-ionol for male Bactrocera latifrons (Diptera: Tephritidae).  Biochem. Systematics and Ecology 35(4):188-195.

116.  Wang, D.; Atkinson, S.; Hoover-Miller, A.; Lee, S.-E.; Li, Q.X.2007.  Organochlorines in harbor seal (Phoca vitulina) tissues from the northern Gulf of Alaska.  Environ/ Pollut. 146:268-280.

103.  Lee, S.-E.; Li, Q.X.; Yu, J.  2006.  Proteomic responses to formic acid on Ralstonia eutropha. Proteomics 6:4259-4268.

99.  Harada, R.M.; Campbell, S.; Li, Q.X.  2006.  Pseudoxanthomonas kalamensis sp. nov., a novel gammaproteobacterium isolated from Johnston Atoll, North Pacific Ocean.  Int J Syst Evol Microbiol. 56:1103-1107.

97.  Rui, Yu-Kui; Yi, Guo-Xiang; Zhao, Jing; Wang, Bao-Min; Li, Zhao-Hu; Zhai, Zhi-Xi; He, Zhong-Pei; Li, Qing X.  2005.  Changes of Bt toxin in the rhizosphere of transgenic Bt cotton and its influence on soil functional bacteria.  World J. Microbiol. & Biotechnol. 21(6):1279-1284.

95.  Cho, I.-K.; Chang, C.L.; Li, Q.X.  2005.  Nicotinamide in relation to dietary nicotinic acid and nine other vitamins and larval development of Ceratitis capitata (Diptera: Tephritidae). J. Agric. Food Chem.  53:7307-7311.

88.  Wang, D.; Atkinson, S.; Hoover-Miller, A.; Li, Q.X.2005.  Analysis of organochlorines in harbor seal tissue samples from Alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique.  Rapid Comm. in Mass Spectrom. 19(13): 1815-1821.

86.  Pelleguer, J.-L.; Chen, S.-W. W.; Karu, A.E.; Li, Q.X.; Roberts, V.A.  2005.  Structural basis for preferential binding of non-ortho-substituted polychlorinated biphenyls by the monoclonal antibody S2B1.  J. Mol. Recog. 18:282-294. 

85.  Shelver, W.L.; Keum, Y.-S.; Kim, H.-J.; Rutherford, Drew; Hakk, Heldur H.; Bergman, Ake; Li, Q.X.  2005.  Hapten syntheses and antibody generation for the development of polybrominated flame retardants ELISA.  J. Agric. Food Chem.  53(10): 840-3847.

83.  Delanoy, G.; Li, Q.X.; Yu, J.  2005.  Activity and stability of laccase in conjugation with chitosan.  Int’l J. of Biological Macromolecules.  35(1-2): 89-95.

82.  Keum, Y.-S.; Li, Q.X.  2005.  Reductive debromination of polybrominated diphenyl ethers by zero-valent Iron.  Environ. Sci. Technol.  39(7):2280-2286.

81.  Hou, S. et al.  2004.  Genome sequence of the deep-sea γ-Proteobacterium Idiomarina Ioihiensis reveals amino acid fermentation as source of carbon and energy.  Proc Natl Acad Sci USA.  101(52): 18036-18041.

75.  Keum, Y.-S.; Li, Q.X.  2004.  Copper dissociation as a mechanism of fungal laccase denaturation by humic acid.  Appl. Microbiol. Biotech. 64: 588-592.

70.  Paquin, D.; Campbell, S.; Li, Q.X.  2004.  Phytoremediation in sub-tropical Hawaii– a review of over 100 plant species.  Remediation. 14(2): 127-139.

67.  Willcox, M.K.; Woodward, L.; Ylitalo, G.; Buzitis, J.; Atkinson, S. Li, Q.X.  2004.  Survey for organochlorines in the free-ranging Hawaiian monk seal (Monachus schauinslandi) at French Frigate Shoals, North Pacific Ocean.  Sci. Total Environ. 322(1-3): 81-93.

57.  Zhu, Y.; Li, Q.X.  2002.  Movement of bromacil and hexazinone in soils of Hawaiian pineapple fields. Chemosphere. 49(6): 671-676.

51.  Chiu, Y.-W.; Li, Q.X.; Karu, A.E.  2001.  Selective binding of polychlorinated biphenyl congeners by a monoclonal antibody: analysis by kinetic exclusion fluorescence immunoassay. Anal. Chem.  73(22):5477-5484.

46.  Pellequer, J.L.; Zhao, B.; Kao, H.-I.; Bell, C.W.; Karu, A.E.; Li, K.; Li, Q.X.; Roberts, V.A.  2000.  Stabilization of bound polycyclic aromatic hydrocarbons by a p-cation interaction. J. Mol. Biology. 302(3):691-699.

45.  David, M.D.; Campbell, S.; Li, Q.X.  2000.  Pressurized fluid extraction of polar herbicides, and polar herbicides using in-situ derivatization.  Anal. Chem. 72(15):3665-3670.

42.  Thomas, S.D.; Li, Q.X. 2000.  Immunoaffinity chromatography for the analysis of polycyclic aromatic hydrocarbons in corals. Environ. Sci. & Technol. 34(12), 2649-2654.

39.  Chiu, Y.-W.; Chen, R.; Li, Q.X.; Karu. A.E.  2000. Derivation and properties of recombinant Fab antibodies to coplanar polychlorinated biphenyls. J. Agric. Food Chem. 48 (6):2614-2624.

32.  Liu, M.; Li, Q.X.; Rechnitz, G.A.  2000.  Gold electrode modification with thiolated hapten for the design of amperometric and piezoelectric immunosensors.  Electroanalysis 12:21-26.

29.  Guo, F.; Li, Q.X.; Alcantara-Licudine, J.P.  1999.  A simple Na4EDTA-assisted sub/supercritical fluid extraction procedure for quantitative recovery of polar analytes in soil.  Anal. Chem. 71:1309-1315.

27.  Li, K.; Chen, R.; Zhao, B.; Liu, M.; Karu A.E.; Roberts, V.A.; Li, Q.X.  1999.  Monoclonal antibody-based ELISAs for part-per-billion determination of polycyclic aromatic hydrocarbons: effects of haptens and formats on sensitivity and specificity. Anal. Chem  71(2):302-309.

15.  Li, Q.X.; Casida, J.E.  1994.  Structure-activity studies leading to potent chloride channel blockers: 5e-tert-butyl-2-[4-(substituted-ethynyl)phenyl]-1,3-dithianes.  Bioorg. & Med. Chem., 2(12):1423-1434.

5.  Li, Q.X.; Gee, S.J.; McChesney, M.M.; Hammock, B.D.; Seiber, J.N.  1989.  Comparison of an enzyme-linked immunosorbent assay and a gas chromatographic procedure for the determination of molinate residues.  Anal. Chem., 61:818-823.